After the run is finished, load the IDLE.M method. Always either ignore this message (it will go away at the end of the solvent delay) or select NO!!! 5. Once the run is started, a window appears that says “Override solvent delay”. Vial # where you have placed your sample. The data path must exist before you start the run or you will get an error message that says “Path must exist” and the run will not start. Use the browse function to select and/or create sub-directories under your username. Save your data in the D:\DATA\ directory. Specify the following parameters in the run window. Select the “Run” icon in the “Method” section. Collecting data for a single sample (Running a method). Method Print method check boxes next to GC/ALS and MS Parameters OK.Ĭ.
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3) You may also print a copy of the method for your records.
![agilent chemstation editing scan parameter threshold agilent chemstation editing scan parameter threshold](https://demo.pdfslide.net/img/380x512/reader024/reader/2021010813/5ae6b9e37f8b9a29048de598/r-1.jpg)
2) Otherwise, specify a different name and include your name in the method name. 1) If you have only changed the inlet temperature and oven parameters that have been discussed above, it is OK to overwrite the method named “Auto_Generic.M”. Define the ions that make up each group (m/z, dwell time). In the “Plot window #2 section, input the low mass and the high mass of a range that covers at least 1 amu. Set up the mass range under the “Plotting” tab. If you change the solvent delay, do not overwrite Auto_Generic.M. The delay is required to avoid overloading the mass spectrometer with the solvent as it moves off the column. This means that the MS detector will turn on 3 minutes after the injection.
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Sample concentration - The sample concentration should be 100˚C, change the Solvent Delay from 3 minutes to 4 minutes. The most commonly used solvents are ethyl acetate, dichloromethane, chloroform, hexanes and diethyl ether. Water is detrimental to the GC column so make sure that the solvent is very dry. 110oC) may not fully evaporate and should be avoided. Analysis of Liquid Samples on the Agilent GC-MS I.